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FREEZING AND STORAGE
OF SPERM
WHAT
DOES THIS INVOLVE?
The freezing of sperm has been performed
since the early 1950’s. A semen sample, is
mixed with a cryoprotectant (similar to antifreeze in a car) and drawn
into thin plastic straws, each of about ½ cc in volume. Normally
between 5 and 20 straws may be prepared from each single ejaculate.
The straws are coloured and a coloured plug is used to seal the end.
These colour combinations are unique for each man. The man’s file
number and sample number is also recorded on each straw. These straws
are then placed into a programmable freezing machine and liquid is pumped
into it at a controlled rate. This allows the sperm straws to be frozen
in a scientifically controlled manner.
At the completion of the freezing programme,
the straws of semen are stored in liquid nitrogen at -196 C in a state
of suspended animation. Sperm can stay in this situation for many years
without any detrimental effect.
WHO WOULD STORE SPERM?
The freezing and storage of sperm is
undertaken for many reasons:
Pre-vasectomy / Pre-chemotherapy
Men may store sperm before undergoing
vasectomy (chemotherapy) as an insurance policy. Vasectomies (chemotherapy)
should be considered as irreversible because repair and reconnection
of the vas some time after vasectomies (chemotherapy) usually is accompanied
by a significant reduction in normal fertilizing capacity of sperm.
In some countries, the Clinic cannot
accept a consent relating to the usage of his semen after the event
of his death and is not permitted to carry out a posthumous (after death)
procedure using his gametes (sperm).
Sperm Donation
Sperm donation may be anonymous or may
be provided to know recipients. The storage of sperm allows artificial
insemination to be performed without the complication of managing the
sperm collection procedure. Sperm donors are also health tested and
sperm sample remain in quarantine for six (6) months awaiting final
health tests on donors before release for use. This eliminates the risk
of transfer of the known viral diseases.
Management of Assisted
Reproductive Treatment
Some men who are not going to be readily
available when their partners are undergoing treatment for assisted
conception or men who find semen collection on demand a difficult exercise,
could consider the storage of sperm a convenient option.
MANAGEMENT OF FREEZE
/ STORAGE OF SPERM
All men who wish to store sperm need
to undergo blood tests for HIV, Hepatitis B and Hepatitis C.
Consent forms to store semen must be
signed by men before storage, and this consent will last for a defined
number of years.
To collect their sperm sample men need
to book with the laboratory. Men need to normally abstain from sexual
relations for 3 days prior to collection.
If there is any concern from the laboratory
about the quality of the sperm, men will be informed of this.
USE OF SPERM
The sperm will be thawed and used only
with specific written consent of the man who owns the stored semen.
Men who choose to donate their sperm have the right to vary or withdraw
their consent at any time.
This page was last updated on September 17, 2001
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